Friday, September 14, 2012

Sept. 17, 2012



Per base quality graph

Topic: Quality filtering NGS data

Discussion leader:  Beth

Papers: Dohm et al. 2008
            Kircher et al. 2011

Discussion Questions

Software: FastQC

Datasets: Illumina reads 1, Illumina reads 2

Artifacts of random hexamer priming at 5' read ends

Note: Download and install FastQC on your computer. I'll be posting some Illumina read sets for you to quality-check, and we'll discuss the results on Monday. -Christina



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