April 10, 2013

Topic: Population scale sequencing
Discussion Leader: Louis
Paper: Lamichhaney et al. 2013

Supplementary information

March 27, 2013

Topic: QTL-seq
Discussion Leader: Christina
Paper: Tagaki et al. 2013
Discussion questions

March 13, 2013

Topic: Single molecule bar-coding in RNAseq

Discussion leader:  Douglas

Paper: Shiroguchi_2012
Supplementary paper: Fu et al. 2011
Discussion Questions

Feb. 27, 2013

Topic: Long non-coding RNAs

Discussion leader:  Christina

Papers:  Sigova et al. 2013
             Supplementary data

Discussion questions

Sept. 17, 2012

Topic: Quality filtering NGS data

Discussion leader:  Beth

Papers: Dohm et al. 2008
            Kircher et al. 2011

Discussion Questions

Software: FastQC

Datasets: Illumina reads 1, Illumina reads 2

Artifacts of random hexamer priming at 5' read ends

Note: Download and install FastQC on your computer. I'll be posting some Illumina read sets for you to quality-check, and we'll discuss the results on Monday. -Christina

Sept. 3, 2012

Topic: NGS Platforms

Discussion leader: Kristine

Papers: Metzker et al 2010
            Hudson 2008

Discussion questions